It does make a difference. I have never used auto line view, or if I have I've forgotten precisely how it works. One line view presents you with what most people are used to: date-time stamp, sender, subject, and a couple more fields. Two line will give you the same plus whatever can be displayed of the first line of the e-mail message; it's sort of a "micro preview."
It certainly won't hurt to try each just to see what you get when that view is in use. You can always switch back to whichever one you prefer.
Brian - Windows 10 Home, 64-Bit, Version 1803, Build 17134
A great deal of intelligence can be invested in ignorance when the need for illusion is deep.
~ Saul Bellow, To Jerusalem and Back